Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / En Face Endocardial Cushion Preparation for…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology
En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue (Video) | JoVE
Journal (Developmental Biology)
Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue (Video) | JoVE
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
Journal (Developmental Biology)
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
 

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Article DOI: 10.3791/64207-v • 08:57 min • July 27th, 2022
July 27th, 2022 •
1,2, 1,2, 1,2
1Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares

Chapters

Summary
Automatic Translation

Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

Tags

En Face Endocardial Cushion Preparation Planar Morphogenesis Analysis Mouse Embryos Primitive Endocardium Distribution Of Endocardial Cells Anisotropy Of Other Injunctions Shape Of Single Endocardial Cell Valve Development Tissue Sections Inner Valve Regenerative Region Planar Cell Polarity Dynamics Of Endocardial Cells Intact Embryos Cardio Development Uterus Excised From Pregnant Mice Ice-cold PBT Decidua Removal Genotyping Yolk Sac Tail Digestion 4% PFA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter