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Primary Culture of Porcine Retinal Pigment Epithelial Cells

Article DOI: 10.3791/64244-v • 07:59 min • September 23rd, 2022
September 23rd, 2022 •
*1,2,3, *1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, School of Medicine, Xiamen University, 2Department of Ophthalmology, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, 3Xiamen University Affiliated Xiamen Eye Center, School of Medicine, Xiamen University
* These authors contributed equally

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Summary
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Here, an easy-to-follow method to culture primary porcine retinal pigment epithelial cells in vitro is presented.

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Primary Culture Porcine Retinal Pigment Epithelial Cells RPE RPE Related Disorders Virtual Culture Physiological Studies Pathological Studies Easy Protocol RP Characteristics Macistate Studies Drug Screenings Treatment For RPE Disorders Value Demonstration Replicate In Different Laboratories Tissue Digestion Enzyme Malaquad 1X PBS Penicillin Streptomycin Culture Plate Transwell Inserts Laminin Solution Heliciculture Incubator
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