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Autofluorescence Imaging to Evaluate Red Algae Physiology
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Autofluorescence Imaging to Evaluate Red Algae Physiology

Article DOI: 10.3791/64533-v • 05:54 min • February 17th, 2023
February 17th, 2023 •
1, 2,3, 4
1Microscopy Core Facility, Technological Research and Scientific Area (ACTI), University of Murcia, 2Confocal Microscopy and Cellular Imaging Unit, Genetic and Molecular Medicine Department, Pediatric Institute for Rare Diseases, Hospital Sant Joan de Déu, 3Research Institute Sant Joan de Déu, Esplugues de Llobregat, Spain 4Algology Laboratory, Biology Faculty, University of Murcia, 4Algology Laboratory, Biology Faculty, University of Murcia

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Summary
Automatic Translation

The present protocol describes step-by-step autofluorescence imaging and evaluation of phycobiliprotein changes in red algae based on spectral analysis. This is a label-free and non-destructive method to evaluate cellular adaptation to extreme habitats, when only scarce material is available and cells grow slowly, or not at all, under laboratory conditions.

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Autofluorescence Imaging Red Algae Physiology Confocal Microscopy Ecological Behavior Microalgae Extreme Environments Pigments Non-invasive Technique Chemical Compounds Autotrophs Growth Performance Control Methods Touristic Caves Architectural Monuments Chroothece Mobilis Inoculum Preparation Seawater Medium SWES Liquid Medium Light-dark Photo Period Cell Density Exponential Phase Culture 24-well Plate Monochromatic Light Effect Green Filter Red Light Filter Confocal Laser Scanning Microscopy
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