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Autofluorescensavbildning for å evaluere rødalgefysiologi

Article DOI: 10.3791/64533-v • 05:54 min • February 17th, 2023
February 17th, 2023 •
1, 2,3, 4
1Microscopy Core Facility, Technological Research and Scientific Area (ACTI), University of Murcia, 2Confocal Microscopy and Cellular Imaging Unit, Genetic and Molecular Medicine Department, Pediatric Institute for Rare Diseases, Hospital Sant Joan de Déu, 3Research Institute Sant Joan de Déu, Esplugues de Llobregat, Spain 4Algology Laboratory, Biology Faculty, University of Murcia, 4Algology Laboratory, Biology Faculty, University of Murcia

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Denne protokollen beskriver trinnvis autofluorescensavbildning og evaluering av fykobiliproteinendringer i rødalger basert på spektralanalyse. Dette er en etikettfri og ikke-destruktiv metode for å evaluere cellulær tilpasning til ekstreme habitater, når bare knappe materialer er tilgjengelige og celler vokser sakte, eller ikke i det hele tatt, under laboratorieforhold.

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Biologi utgave 192 autofluorescens fykobiliner klorofyll konfokal laserskanningsmikroskopi (CLSM) rødalger Chroothece
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