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JoVE Journal Bioengineering
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Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture

Article DOI: 10.3791/64554-v • 08:11 min • October 28th, 2022
October 28th, 2022 •
1, 2
1Department of Biomedical Engineering, Duke University, 2Departments of Biomedical Engineering, Neurology, and Dermatology, Duke University

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Summary
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Minimizing the variability in the particle fraction within granular scaffolds facilitates reproducible experimentation. This work describes methods for generating granular scaffolds with controlled particle fractions for in vitro tissue engineering applications.

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Particle Fraction Microporous Annealed Particle Scaffolds 3D Cell Culture Granular Materials Hydrogel Particles Material Properties Cellular Responses Scaffold Properties Cell Responses Repair And Regeneration Drug Delivery Cellular Infiltration Regeneration Granular Hydrogels User-controlled Particle Fractions Bioactivity Unique Cell Responses Microfluidic Microgel Generation Hyaluronic Acid (HA) Norbornene (NB) Cryo-safe Screw-cap Tube Positive Displacement Pipette
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