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JoVE Journal Neuroscience
Påvisning av unormalt prionprotein ved immunhistokjemi
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Påvisning av unormalt prionprotein ved immunhistokjemi

Article DOI: 10.3791/64560-v • 06:38 min • May 5th, 2023
May 5th, 2023 •
1,2, 1, 2, 2, 2, 3, 2, 1, 1, 1, 1, 1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão

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Immunmerking av unormalt prionprotein ved bruk av immunhistokjemiske protokoller krever spesifikke prøve- og anti-PrP-antistoffpreparasjonsmetoder. Den nåværende protokollen beskriver de viktigste trinnene i epitopdemasking for å sikre riktig PrP-immunmerking og for å minimere uspesifikk bakgrunnsfarging. Også denne tilnærmingen vurderer biosikkerhetstiltak når man utfører immunhistokjemiske studier med prioninfiserte vev.

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