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Journal / Neuroscience / Detektion av onormalt prionprotein genom…
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JoVE Journal Neuroscience
Detektion av onormalt prionprotein genom immunhistokemi
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Detektion av onormalt prionprotein genom immunhistokemi

Article DOI: 10.3791/64560-v • 06:38 min • May 5th, 2023
May 5th, 2023 •
1,2, 1, 2, 2, 2, 3, 2, 1, 1, 1, 1, 1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão

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Immunolabeling onormalt prionprotein med hjälp av immunhistokemiska protokoll kräver specifika prov- och anti-PrP-antikroppsberedningsmetoder. Detta protokoll beskriver de viktigaste stegen i epitopdemaskering för att säkerställa korrekt PrP-immunmärkning och för att minimera icke-specifik bakgrundsfärgning. Detta tillvägagångssätt beaktar också biosäkerhetsåtgärder vid genomförande av immunhistokemiska studier med prioninfekterade vävnader.

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Neurovetenskap nummer 195
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