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In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Article DOI: 10.3791/64718-v • 05:36 min • February 10th, 2023
February 10th, 2023 •
1,2, 1,3
1Lester & Sue Smith Breast Center, Baylor College of Medicine, 2Department of Medicine, Baylor College of Medicine, 3Department of Molecular & Cellular Biology, Baylor College of Medicine, Houston, TX 77030

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Summary
Automatic Translation

The present protocol describes intraductal injection of viral vectors via the teat to deliver genes of interest into the mammary epithelial cells.

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Gene Delivery Mouse Mammary Epithelial Cells Mammary Intraductal Injection Genetic Material Delivery Mammary Gland Temporary Delivery Method Quantitatively Controllable Delivery Mammary Models Tumor Genesis Syringe Preparation Needle Cutting Alcohol Sterilization Viral Stock Tubes Bromophenol Blue Addition Virus Suspension Mixing Anesthesia Depth Check Ointment Application Supine Position Limb Attachment Nipple Identification
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