JoVE Journal
Cancer Research
Cancer Research
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Here, we describe an immunofluorescence-based method to quantify the levels of single-stranded DNA in cells. This efficient and reproducible method can be utilized to examine replication stress, a common feature in several ovarian cancers. Additionally, this assay is compatible with an automated analysis pipeline, which further increases its efficiency.