Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Evaluating Autophagy Levels in Two Different…
JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
Large-Scale Screens of Metagenomic Libraries
Journal (Biology)
Large-Scale Screens of Metagenomic Libraries
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities
Journal (Biology)
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities
 

Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

Article DOI: 10.3791/65005-v • 08:07 min • April 28th, 2023
April 28th, 2023 •
1, 1, 2, 2, 1
1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, 2Signalling Programme, The Babraham Institute

Chapters

Summary
Automatic Translation

The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.

Tags

Autophagy Levels LC3 Immunofluorescence Pancreatic Cell Models Agents Protein Aggregates Felipe Renna Malena Herrera Lopez Maria Ines Vaccaro Cell Preparation Cover Slips Ultraviolet Radiation DMEM FBS Penicillin Streptomycin Gemcitabine Solution Fixing And Permeabilizing Cells Methanol
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter