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JoVE Journal Engineering
Normothermic Ex Vivo Liver Machine Perfusion in Mouse
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Normothermic Ex Vivo Liver Machine Perfusion in Mouse

Article DOI: 10.3791/65363-v • 13:14 min • September 25th, 2023
September 25th, 2023 •
1, 2, 1,3, 1, 1
1Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Institute of Pathology, Klinikum Chemnitz GmbH, 3Department of Pathology, Faculty of Veterinary Medicine, Menoufia University

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Summary
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A normothermic ex vivo liver perfusion (NEVLP) system was created for mouse livers. This system requires experience in microsurgery but allows for reproducible perfusion results. The ability to utilize mouse livers facilitates the investigation of molecular pathways to identify novel perfusate additives and enables the execution of experiments focused on organ repair.

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Normothermic Ex Vivo Liver Machine Perfusion Mouse Model Molecular Mechanisms Genetically Modified Animals Transgenic Mice Normothermic Oxygenated Machine Perfusion System Thermostatic Controller Perfusate Oxygenator Bubble Trap Portal Vein Suprahepatic Cava Bile Drainage Tube Operating Table Semi-sterile Operating Conditions
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