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JoVE Journal Biology
Culture of myeloid dendritic cells from bone marrow precursors
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Culture of myeloid dendritic cells from bone marrow precursors

Article DOI: 10.3791/769-v • 06:39 min • July 25th, 2008
July 25th, 2008 •
1,2, 2,3, 2, 2
1Medical Sciences Program, McMaster University, 2Centre for Gene Therapeutics, McMaster University, 3Department of Chemical Engineering, University of Waterloo

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Summary
Automatic Translation

This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.

Tags

Myeloid Dendritic Cells Bone Marrow Precursors Immune Mechanisms Antigen Presenting Cells Vaccine Vector Tibias And Femurs In Vitro Differentiation Of DCs Functional Activity Of DCs Cell Surface Markers Cytokine Production Maturation Of DCs Flow Cytometric Comparisons Co-stimulatory Molecules IL-12 Cytokine CpG Stimulation Mock Treatment Vaccine Vector Manipulation Antigen-specific Immune Responses In Vitro Pulsing Recombinant Viral Vectors
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