Journal
/
Biology
/
Microinjection of Zebrafish Embryos to Analyze Gene Function
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Microinjection of Zebrafish Embryos to Analyze Gene Function

Microinjection of Zebrafish Embryos to Analyze Gene Function

DOI:
10.3791/1115-v

•

07:18 min

•

March 09, 2009

•

, ,
1Department of Genetics,Harvard Medical School, 2Department of Cardiology,Children’s Hospital Boston

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:12Zebrafish Egg Collection
  • 02:24Needle Pulling, Loading, and Preparation
  • 04:23Injection
  • 05:48Representative Results: Morpholino and mRNA Injection Phenotypes
  • 06:33Conclusion

Summary

Automatic Translation

Automatic Translation

This video shows how morpholino or mRNA can be injected into zebrafish embryos at the one-cell stage to decrease or increase the level of specific gene products during subsequent development.

Tags

MicroinjectionZebrafish EmbryosGene Function AnalysisProtein ManipulationMorpholinosAntisense OligonucleotidesGene ExpressionMRNA InjectionMicroinjection ProcessEmbryo ManipulationInjection Volume

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1115?article_type=v

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
check_url/1115?article_type=v

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
check_url/1115?article_type=v

Generating Chimeric Zebrafish Embryos by Transplantation
check_url/1115?article_type=v

Using the optokinetic response to study visual function of zebrafish
check_url/1115?article_type=v

Microinjection of Medaka Embryos for use as a Model Genetic Organism
check_url/1115?article_type=v

Metabolic Profile Analysis of Zebrafish Embryos
check_url/1115?article_type=v

Gene Trapping Using Gal4 in Zebrafish
check_url/1115?article_type=v

Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
check_url/1115?article_type=v

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
check_url/1115?article_type=v

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Read Article