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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

DOI:
10.3791/1205-v

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11:56 min

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April 17, 2009

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, , , ,
1Department of Evolutionary Functional Genomics, Evolutionary Biology Center,Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter,Swedish University of Agricultural Sciences

Chapters

  • 00:00Title
  • 00:45Fixation and Embedding
  • 01:41Sectioning
  • 06:16In Situ Hybridization
  • 11:21Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.

Tags

Non-radioactive In Situ HybridizationProtocolNorway SprucePlant SpeciesExpression Analysis TechnologiesExpression ProfilesIn Situ HybridizationMRNA ExpressionLaboriousTime-consumingOptimizationWoody SpeciesConiferPicea AbiesModified DIG In Situ Hybridization ProtocolRNase TreatmentProteinase K ConcentrationHomologous GenesMale Reproductive OrgansGymnospermAngiosperm SpeciesArabidopsis ThalianaBrassica NapusFloral OrgansMicrosporophylls

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