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Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
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Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

DOI:
10.3791/1292-v

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06:34 min

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June 10, 2009

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, ,
1Plant Pathology and Plant-Microbe Biology,Cornell University, 2Boyce Thompson Institute for Plant Research

Chapters

  • 00:05Title
  • 00:55Introduction
  • 01:22Preparing Agrobacterium tumefaciens for Viral Delivery
  • 04:26Viral Infiltration of Nicotiana benthamiana and Tomato Plants
  • 05:38Representative Results: PDS Silenced Plants
  • 06:06Conclusion

Summary

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Automatic Translation

Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.

Tags

Virus-induced Gene SilencingVIGSNicotiana BenthamianaTomatoRNA InterferenceRNAiGene-silencing PhenomenonDsRNAMicroRNAsShort Interfering RNAsTransposonsDevelopmental ProcessesFlower Organ FormationLeaf DevelopmentViral InfectionSuppressors Of Gene SilencingColonizationAntiviral Defense MechanismPlant Pathogen Agrobacterium TumefaciensTi PlasmidRecombinant VirusFunctional GenomicsRibonuclease Dicer

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