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Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
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Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes

Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes

DOI:
10.3791/1631-v

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14:23 min

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December 04, 2009

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,
1Department of Neuroscience,University of Pennsylvania-School of Medicine, 2Department of Physiology,University of Pennsylvania-School of Medicine

Chapters

  • 00:00Title
  • 00:59Introduction
  • 01:46Tissue Preparation
  • 05:39Preparation of Tissue Samples for Optical Recording
  • 07:10Optical Recording of Electrical Activity
  • 10:30Image Analysis
  • 12:00Representative Optical Data
  • 13:14Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol illustrates how voltage-sensitive dyes enable optical recording of electrical activity from intact neural networks such as the plexuses of the guinea-pig enteric nervous system, with an adjustable resolution that ranges from single-cells to multi-ganglionic circuitry.

Tags

Optical RecordingElectrical ActivityGuinea-pig Enteric NetworksVoltage-sensitive DyesEnteric Nervous SystemMyenteric PlexusSubmucous PlexusReflex PathwaysGangliaSynaptic InteractionsGastrointestinal FunctionExperimental ModelMolecular EventsCellular EventsNeuronal NetworkPhysiological Outputs

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