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Plastic Embedding and Sectioning of Xenopus laevis Embryos
JoVE Journal
Biology
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JoVE Journal Biology
Plastic Embedding and Sectioning of Xenopus laevis Embryos
DOI:

14:39 min

April 29, 2007

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Chapters

  • 00:11Introduction
  • 00:55Important Tools
  • 01:37Embryo dissection
  • 04:13Embedding
  • 09:15Sectioning and mounting
  • 12:58Examples of results obtained

Summary

Automatic Translation

Plastic sections maintain true tissue morphology in thin sections of tissue that can be immunostained with fluorescent secondary antibodies, making this method more useful than paraffin-embedded or frozen sections for many types of tissue. The method for staining, plastic embedding, and sectioning is demonstrated in this video.

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