Journal
/
Immunology and Infection
/
Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

DOI:
10.3791/2221-v

•

06:13 min

•

November 23, 2010

•

, , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Department of Microbiology and Immunology,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 00:58Coating the ELISPOT Plate
  • 01:45Cell Preparation
  • 02:08Setting up the Plate
  • 03:25Developing the ELISPOT Plate
  • 05:53Conclusion

Summary

Automatic Translation

Automatic Translation

Identification of microbial targets of adaptive immunity in idiopathic diseases can be accomplished by the use of the enzyme-linked immunospot assay.

Tags

Enzyme-linked Immunospot AssayELISPOTCellular Immune ResponsesMicrobial AntigensTh-1 Immune ResponseDiagnostic ToolImmunosuppressed PopulationsHIV InfectionTransplantationSteroid UseTh1 Or Th2 Immune ResponsePeripheral BloodBronchoalveolar LavageCerebral Spinal FluidAscitesEpitopes Within Microbial ProteinsImmune Responses Over TimeDual Color ELISPOT AssaysCytokinesExtrapulmonary TuberculosisInfectious AntigensAutoimmunity

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2221?article_type=v

Seven Steps to Stellate Cells
check_url/2221?article_type=v

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
check_url/2221?article_type=v

Concurrent Quantification of Cellular and Extracellular Components of Biofilms
check_url/2221?article_type=v

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
check_url/2221?article_type=v

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
check_url/2221?article_type=v

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
check_url/2221?article_type=v

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
check_url/2221?article_type=v

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
check_url/2221?article_type=v

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
check_url/2221?article_type=v

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Read Article