JoVE Journal
Biology
Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Calcium is a ubiquitous messenger in the nervous system, essential for triggering neurotransmitter release and changes in synaptic strength. Here we demonstrate a technique for loading Ca2+-indicators into Drosophila nerve terminals. We also demonstrate fabrication of the required apparatus and emphasize points critical for the technique's success.