Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Keiji Mochida; Ayumi Hasegawa; Kyuichi Taguma; Atsushi Yoshiki; Atsuo Ogura

doi:10.3791/3155

Journal

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Biology

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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

DOI:

10.3791/3155-v

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06:00 min

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November 18, 2011

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Keiji Mochida, Ayumi Hasegawa, Kyuichi Taguma, Atsushi Yoshiki, Atsuo Ogura

¹RIKEN BioResource Center

Chapters

00:05Title
01:13Vitrification of 2-cell Mouse Embryos
02:22Thawing Vitrified 2-cell Mouse Embryos
05:03Representative Results
05:30Conclusion

Summary

Automatic Translation

An ethylene glycol-based vitrification method for mouse embryos is described. It is advantageous to other methods in its simplicity and low embryonic toxicity, and therefore can be broadly applicable to many strains of mice, including inbred and gene-modified mice.

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

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