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Chapters
- 00:05Title
- 02:01DNA Preparation for Transgenesis
- 02:51Microinjection into Hermaphroditic Gonads
- 05:06Post-injection Storage & Screening
- 05:45The P. pacificus Model
- 06:26Conclusion
In model organisms, transgenesis can manipulate gene functions while RNAi can knockdown specific mRNA transcripts 1-2. This protocol aims to illustrate the techniques needed to introduce stably transmitted DNA and transient double stranded RNA into the necromenic nematode Pristionchus pacificus for studies in evolutionary, developmental, and behavioral biology.
Tags
RNAi Mediated Gene KnockdownTransgenesisMicroinjectionNecromenic NematodePristionchus PacificusGene Function AnalysisExpression AnalysisCaenorhabditis ElegansTransgenic LinesNon-Caenorhabditis SpeciesNematodaStrongyloides StercoralisDevelopment StudyEvolution StudyBehavior StudyMicroinjection For TransgenesisGene Knockdown By RNAiGonads Of P. PacificusSyncitial GonadsTransgene InheritanceSomatic ExpressionSelf Genomic DNAEndonucleasesCoinjection Markers
