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RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
This content is Free Access.
Chapters
- 00:05Title
- 01:21Harvesting Murine Luminal Contents
- 02:44Bacterial RNA Isolation
- 06:03DNAse Treatment
- 07:48Representative Agilent Biolanalyzer Analysis of Murine Cecal Content-extracted Bacterial RNA
- 08:35Conclusion
A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.
Tags
RNA IsolationPseudomonas AeruginosaGastrointestinal TractColonizationImmune SystemsLeukemiaBurn WoundsOrgan TransplantsBloodstream InfectionsTranscription ProfilingMRNAGene ExpressionImmune StatusIsolationHigh Quality MRNAEnzymesDebrisFood ResiduesParticulate MatterGI ColonizationNeutropenia-induced DisseminationMice Euthanization
