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Chapters
- 00:05Title
- 01:57Preparation of Mammary Architecture and Microenvironment Engineering (MAME) Cocultures
- 04:08Performing 4D (3D + time) Imaging of Live MAME Cocultures by Confocal Microscopy
- 05:11Representative 4D MAME Live-cell Imaging Models
- 07:57Conclusion
We have developed 3D coculture models for live-cell imaging in real-time of interactions among breast tumor cells and other cells in their microenvironment that impact progression to an invasive phenotype. These models can serve as preclinical screens for drugs to target paracrine-induced proteolytic, chemokine/cytokine and kinase pathways implicated in invasiveness.
Tags
MAME Models4D Live-cell ImagingTumor Microenvironment InteractionsMalignant ProgressionCoculture ModelsPreinvasive Breast LesionsInvasive PhenotypeBreast Epithelial CellsTumor MicroenvironmentMyoepithelial CellsFibroblastsMacrophagesBlood And Lymphatic Microvascular Endothelial CellsProstate Cancers3D CoculturesLive-cell ImagingFunctional Proteolysis AssayBreast Ductal Carcinoma In Situ (DCIS) Cells
