Journal
/
Bioengineering
/
Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish
JoVE Journal
Bioengineering
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

DOI:
10.3791/3780-v

•

10:32 min

•

September 30, 2012

•

, ,
1Neurobiology,NCBS-TIFR, 2Department of Biological Sciences,TIFR

Chapters

  • 00:00Title
  • 00:39Introduction
  • 02:36SU8 Master Fabrication
  • 04:20PDMS Mold Fabrication
  • 06:41Using PDMS Membrane
  • 07:50Inserting C. elegans, Drosophila and Zebrafish Larvae
  • 09:59Conclusions

Summary

Automatic Translation

Automatic Translation

A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport. We demonstrate the use of this device and show examples of different types of data collected from different model systems.

Tags

Microfluidic DevicesIn Vivo ImagingC. ElegansDrosophilaZebrafishSoft Lithography TechniquesSub-cellular ImagingLaser MicrosurgeryCellular ImagingImmobilization TechniquesSuctionTapered ChannelsDeformable MembranesAnesthetic-free ImmobilizationPDMS DeviceGenetic Model Organisms

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3780?article_type=v

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
check_url/3780?article_type=v

Viral Nanoparticles for In vivo Tumor Imaging
check_url/3780?article_type=v

A Microfluidic Technique to Probe Cell Deformability
check_url/3780?article_type=v

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
check_url/3780?article_type=v

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
check_url/3780?article_type=v

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
check_url/3780?article_type=v

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
check_url/3780?article_type=v

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
check_url/3780?article_type=v

A Simple and Scalable Fabrication Method for Organic Electronic Devices on Textiles
check_url/3780?article_type=v

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

Read Article