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Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
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Chapters
- 00:05Title
- 01:30Fungal Culture Preparation
- 03:13Zebrafish Infections
- 08:25Preparing the Fish for Imaging
- 09:24Results: Zebrafish Hindbrain Ventricle Infection with Candida albicans
- 10:12Conclusion
The rapid development, small size and transparency of zebrafish are tremendous advantages for the study of innate immune control of infection1-4. Here we demonstrate techniques for infecting zebrafish larvae using the fungal pathogen Candida albicans by microinjection, methodology recently used to implicate phagocyte NADPH oxidase activity in control of fungal dimorphism5.
Tags
Non-invasive ImagingDisseminated CandidiasisZebrafish LarvaeCandida AlbicansInnate ImmunityGenetic DefectsPhagocyte NADPH OxidaseDynamics Of C. Albicans InteractionMacrophagesNeutrophilsIn Vitro StudiesIn Vivo EnvironmentCytokine LevelsExtracellular Matrix AttachmentsIntercellular ContactsModel OrganismLive Intact HostZebrafish LarvaInnate Immune Defenses
