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Chapters
- 00:00Title
- 00:13Introduction
- 01:07Two Techniques for the Orthotopic Mouse Model of Colorectal Cancer
- 01:50Instruments
- 02:49Mouse Preparation, Laparotomy, and Exposure of Cecum
- 04:37Injection of Cells into Cecal Wall
- 05:07Transplantation of Tumor onto Cecum
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
Tags
Orthotopic Mouse ModelColorectal CancerSubcutaneous Tumor ModelCancer CellsNatural LocationHuman DiseaseHigh FidelityTechniquesMouse AnesthesiaLaparotomyCecumColorectal Cancer Cell SuspensionSingle Cell SuspensionInjectionLeakageTransplantationSubcutaneous TumorEuthanizedSterile TechniqueTumor PieceTumor Cell InfiltrationPrimary TumorsLiver MetastasesTherapeutic Agents
