Journal
/
Biology
/
C. elegans Chemotaxis Assay
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
C. elegans Chemotaxis Assay

C. elegans Chemotaxis Assay

DOI:
10.3791/50069-v

•

06:28 min

•

April 27, 2013

•

, ,
1Life Sciences,Queen’s University , 2Department of Chemical Engineering,Queen’s University , 3Department of Biology,Queen’s University

Chapters

  • 00:05Title
  • 01:06Preparing the Test Plates
  • 01:45Preparing the Worms
  • 03:27Running the Assay
  • 05:29Comparing Wild-type (N2) C. elegans to the odr-10(ky10) Mutant
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

A method of quantitatively evaluating the chemotactic response of Caenorhabditis elegans is described. A chemotactic index (CI) was employed as a way to precisely evaluate the response of worms to certain targets, and serve as a platform of comparison between strains and compounds of interest.

Tags

C. ElegansChemotaxis AssayOdorant ResponseFour-quadrant SystemWorm BehaviorSample SizeOptimizationBargmann Et Al. Method

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50069?article_type=v

Generation of Stable Transgenic C. elegans Using Microinjection
check_url/50069?article_type=v

Application of a C. elegans Dopamine Neuron Degeneration Assay for the Validation of Potential Parkinson’s Disease Genes
check_url/50069?article_type=v

Dissecting and Recording from The C. Elegans Neuromuscular Junction
check_url/50069?article_type=v

Generation of Transgenic C. elegans by Biolistic Transformation
check_url/50069?article_type=v

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
check_url/50069?article_type=v

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
check_url/50069?article_type=v

Basic Caenorhabditis elegans Methods: Synchronization and Observation
check_url/50069?article_type=v

A Method for Culturing Embryonic C. elegans Cells
check_url/50069?article_type=v

Antibody Staining in C. Elegans Using "Freeze-Cracking"
check_url/50069?article_type=v

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay

Read Article