This content is Free Access.
Chapters
- 00:05Title
- 01:46Yeast Preparations
- 02:51PlateSlide Preparations
- 03:34Microscope Preparations
- 04:58Imaging
- 06:18Results: Modeling Protein Misfolding with GFP-Ubc9ts
- 08:23Conclusion
Cellular viability depends on timely and efficient management of protein misfolding. Here we describe a method for visualizing the different potential fates of a misfolded protein: refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9ts, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.
