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The MultiBac Protein Complex Production Platform at the EMBL
 

The MultiBac Protein Complex Production Platform at the EMBL

Article DOI: 10.3791/50159-v • 13:51 min • July 11th, 2013
July 11th, 2013 •
1, 1, 1, 1, 1, 1
1EMBL Grenoble Outstation and Unit of Virus Host Cell Interactions (UVHCI) UMR5322

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Summary
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Protein complexes catalyze key cellular functions. Detailed functional and structural characterization of many essential complexes requires recombinant production. MultiBac is a baculovirus/insect cell system particularly tailored for expressing eukaryotic proteins and their complexes. MultiBac was implemented as an open-access platform, and standard operating procedures developed to maximize its utility.

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Keywords: Protein Complex Eukaryotic Proteome Baculovirus Expression Vector System BEVS MultiBac Recombinant Protein Production Multiprotein Assembly Standard Operating Procedures EMBL Grenoble
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