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A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

DOI:
10.3791/50291-v

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13:44 min

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September 02, 2013

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, ,
1Institute for Behavioral Genetics,University of Colorado Boulder, 2Department of Psychology and Neuroscience,University of Colorado Boulder

Chapters

  • 00:05Title
  • 01:50Virus Storage and Preparation
  • 02:57Surgery and Virus Injection
  • 04:59Light Delivery and In vivo Recording of CHR2 or NpHR-Expressing Neurons
  • 08:38Results: High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
  • 13:06Conclusion

Summary

Automatic Translation

Automatic Translation

The recent development of neuroscience tools that combine genetics and optics, termed "optogenetics", enables control over neural circuit activity with an unprecedented level of spatial and temporal resolution. Here we provide a protocol for integrating in vivo recording with optogenetic manipulation of genetically-defined subsets of prefrontal cortical and subicular pyramidal neurons.

Tags

OptogeneticsNeural CircuitChannelrhodopsin-2HalorhodopsinAdeno-associated VirusDorsal SubiculumPrelimbic RegionPrefrontal CortexPyramidal NeuronIn VivoElectrical ActivityLight DeliveryElectrical Recording

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