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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

DOI:
10.3791/50685-v

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12:36 min

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February 16, 2014

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, ,
1Department of Horticulture,University of Kentucky

Chapters

  • 00:05Title
  • 01:14Growing Bacterial Host (BLT5403) for Titering
  • 02:15Affinity Selection
  • 08:13Titering
  • 09:41Plaque Isolation
  • 10:59Results: Typical Titer and PCR Results for Affinity Selection over an Appropriate Bait
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

Phage display is a powerful technique to capture proteins or protein moieties that interact with an immobilized molecule of interest. Once a decision of the type of phage cDNA library to create and screen has been made, the protocol described here permits efficient affinity selection leading to identification of interactors.

Tags

Keywords: Phage DisplayAffinity SelectionRecombinant Protein BaitsProtein-protein InteractionsCDNA LibraryAffinity Selection ProtocolPhage TiterNegative ControlsAmpliconsFalse PositivesViral Contamination

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