Journal
/
Medicine
/
Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

DOI:
10.3791/50768-v

•

08:08 min

•

October 16, 2013

•

, ,
1Viscogliosi Laboratory in Molecular Genetics of Musculoskeletal Diseases,Sainte-Justine University Hospital Research Center, 2Department of Stomatology, Faculty of Dentistry and Department of Biochemistry, Faculty of Medicine,Université de Montréal

Chapters

  • 00:05Title
  • 02:04PBMC Culture
  • 03:38Treat Cells with Recombinant OPN
  • 05:49Results: Prognosis of Idiopathic Scoliosis
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

A structured protocol is presented for a cell-based assay as a functional test to predict the prognosis of idiopathic scoliosis using cellular dielectric spectroscopy (CDS). The assay can be performed with fresh or frozen peripheral blood mononuclear cells (PBMCs) and the procedure is completed within 4 days.

Tags

Cell-based AssayPrognostic PredictionIdiopathic ScoliosisCellular Dielectric SpectroscopyPeripheral Blood Mononuclear CellsGi ProteinsGs ProteinsOsteopontinPhytohemmaglutininSomatostatinIsoproterenol

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50768?article_type=v

An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)
check_url/50768?article_type=v

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
check_url/50768?article_type=v

The Trier Social Stress Test Protocol for Inducing Psychological Stress
check_url/50768?article_type=v

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
check_url/50768?article_type=v

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
check_url/50768?article_type=v

Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model
check_url/50768?article_type=v

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
check_url/50768?article_type=v

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
check_url/50768?article_type=v

Quantification of Optic Nerve Cross Sectional Area on MRI: A Novel Protocol using Fiji Software
check_url/50768?article_type=v

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement

Read Article