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Journal / Chemistry / Covalent Binding of BMP-2 on Surfaces Using a…
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JoVE Journal Chemistry
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
 

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Article DOI: 10.3791/50842-v • 10:23 min • August 26th, 2013
August 26th, 2013 •
1, 1, 1,2
1Department of Biophysical Chemistry, Institute for Physical Chemistry, University of Heidelberg, 2Max Planck Institute for Intelligent Systems at Stuttgart

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Summary
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We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.

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Keywords: BMP-2 Bone Morphogenetic Protein Self-assembled Monolayer Covalent Immobilization Extracellular Matrix Osteoblast Differentiation In Vitro Cell Studies Growth Factor Presentation
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