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JoVE Journal
Biology

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V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting
 

V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting

Article DOI: 10.3791/50948-v 11:33 min December 30th, 2013
December 30th, 2013

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Summary

V3 workflow is a western blot procedure using stain-free gels. The stain-free technology allows researchers to visualize protein separation quality, to verify the transfer efficiency, and most importantly, to validate the change in the protein of interest using total protein quantification as a reliable loading control.

Tags

Keywords: Western Blot Stain-free Technology Total Protein Loading Control Protein Quantification Workflow Optimization Transfer Efficiency Verification Housekeeping Proteins
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