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JoVE Journal
Bioengineering
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Sample Drift Correction Following 4D Confocal Time-lapse Imaging
 

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Article DOI: 10.3791/51086-v 10:05 min April 12th, 2014
April 12th, 2014

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Summary

Time-lapse microscopy allows the visualization of developmental processes. Growth or drift of samples during image acquisition reduces the ability to accurately follow and measure cell movements during development. We describe the use of open source image processing software to correct for three dimensional sample drift over time.

Tags

Keywords: 4D Confocal Time-lapse Imaging Sample Drift Drift Correction Cell Tracking Fiji ImageJ LOCI Tools Phase Correlation 3D Registration
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