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Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
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Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

DOI:
10.3791/51525-v

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09:03 min

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November 23, 2014

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, ,
1Department of Physiology and Pharmacology, Sackler School of Medicine,Tel Aviv University

Chapters

  • 00:05Title
  • 01:29Extracellular Matrix (ECM)–mimic Gel Plug Inoculation into Mice
  • 02:43Evacuation of ECM–mimic Gel Plug Vascularization Using Ultrasound
  • 05:31Evacuation of ECM–mimic Gel Plug Vasculature Morphology Using Fibered Confocal Endomicroscopy
  • 07:23Results: Representative ECM–mimic Gel Plug Imaging
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a matrigel plug assay to illustrate angiogenic potential of a pool of factors secreted by cancer cells using two complementary imaging modalities, ultrasound and endomicroscopy. The matrigel, an extracellular matrix (ECM)-mimic gel, is utilized to introduce the host (mouse) with angiogenic factors secreted to the conditioned media (C.M.). 

Tags

Matrigel Plug AssayAngiogenesisVasculatureTumor-generating CellsGlioblastomaMicrobubbles Contrast-enhanced Ultrasound ImagingIntravital Fibered-confocal EndomicroscopyPro-angiogenic FactorsAnti-angiogenic FactorsTumor MicroenvironmentCancer Biology

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