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Immunology and Infection

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JoVE Journal Immunology and Infection
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
 

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Article DOI: 10.3791/51647-v • 10:04 min • August 19th, 2014
August 19th, 2014 •
1, 1, 1
1Department of Genetics and Biochemistry, Clemson University Eukaryotic Pathogens Innovation Center

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Summary
Automatic Translation

Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.

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Keywords: Fluorescent Proteins Glycosome Dynamics Trypanosoma Brucei Human African Trypanosomiasis Nagana Peroxisomes Glycolysis Fluorescence Microscopy PTS2-eYFP Flow Cytometry Organelle Composition
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