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Journal
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Biology
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RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue
JoVE Journal
Biology
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JoVE Journal
Biology
RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue
RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue
DOI:
10.3791/51779-v
•
08:47 min
•
August 02, 2014
•
Ana Clara P. Azevedo-Pouly
,
Ola A. Elgamal
,
Thomas D. Schmittgen
1
Pharmaceutics & Pharmaceutical Chemistry
,
The Ohio State University
Chapters
00:05
Title
01:50
Surgery, Pancreas Dissection, and Homogenization
02:57
Removal of Undigested Tissue
04:35
RNA Isolation
06:41
Results: Analysis of RNA Integrity
08:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
We report a procedure to isolate RNA with high integrity from the ribonuclease rich mouse pancreas.
Tags
RNA Isolation
Mouse Pancreas
Guanidine Thiocyanate
RNA Integrity Number (RIN)
Gene Expression Analysis
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