Journal
/
Neuroscience
/
Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

DOI:
10.3791/51853-v

•

08:33 min

•

December 04, 2014

•

, , , , , , , , ,
1Department of Psychiatry,Johns Hopkins University, 2Department of Psychiatry and Behavioral Sciences,Howard University, 3Department of Otolaryngology-Head and Neck Surgery,Johns Hopkins University, 4Department of Psychiatry,Sheppard Pratt Hospital, 5Department of Psychiatry,Indiana University

Chapters

  • 00:05Title
  • 01:49Laser-Capture Microdissection
  • 03:48Isolation of Total RNA and cDNA Synthesis
  • 05:50Real Time PCR Quantification
  • 07:14Reults: GSK-3β Expression in OE Neurons
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, a novel platform to investigate intraneuronal molecular signatures of treatment response in bipolar disorder (BD) was developed and validated. Olfactory epithelium from BD patients was obtained through nasal biopsies. Then laser-capture microdissection was combined with Real Time RT PCR to investigate the molecular signature of lithium response in BD.

Tags

Olfactory NeuronsNasal BiopsyLaser-capture MicrodissectionBipolar DisorderLithium CarbonateWnt SignalingNeuropsychiatric DisordersOlfactory EpitheliumTreatment Response

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51853?article_type=v

Laser Capture Microdissection of Drosophila Peripheral Neurons
check_url/51853?article_type=v

Isolating Nasal Olfactory Stem Cells from Rodents or Humans
check_url/51853?article_type=v

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
check_url/51853?article_type=v

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
check_url/51853?article_type=v

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
check_url/51853?article_type=v

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
check_url/51853?article_type=v

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
check_url/51853?article_type=v

Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
check_url/51853?article_type=v

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons
check_url/51853?article_type=v

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

Read Article