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Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
 

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Article DOI: 10.3791/52041-v • 10:26 min • January 16th, 2015
January 16th, 2015 •
1, 1, 1
1Department of Entomology, Virginia Tech

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A protocol is developed to examine the effects of an epigenetic drug DZNep on the development, fecundity and survivorship of mosquitoes. Here we describe procedures for the aqueous exposure of DZNep to immature mosquitoes and a blood-based exposure of DZNep to adult mosquitoes in addition to measuring SAH hydrolase inhibition.

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Epigenetic Drug Anopheles Gambiae Malaria Vector Toxicological Assay Fecundity Survivorship 3-Deazaneplanocin A DZNep S-adenosylhomocysteine Hydrolase Insecticide Resistance
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