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Developmental Biology
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Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
 

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Article DOI: 10.3791/52769-v 06:00 min May 2nd, 2015
May 2nd, 2015

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Summary

A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.

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Keywords: DNA Methyl Green Fluorescent Labeling Embryos Zebrafish Chick Nuclear Stain Confocal Imaging Photobleaching Histological Stain DNA-specific
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