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The Use of Flow Cytometry to Assess the State of Chromatin in T Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

DOI:
10.3791/53533-v

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11:01 min

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December 17, 2015

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, ,
1Department of Biology,Furman University

Chapters

  • 00:05Title
  • 00:40Single Cell Suspension of Lymphocytes from Mouse Spleens
  • 05:39Viability and Surface Staining
  • 07:55Intracellular Staining for Histone H3
  • 09:42Results: Assessing the State of Chromatin in T Cells
  • 10:30Conclusion

Summary

Automatic Translation

Automatic Translation

Flow cytometry can be utilized to assess the state of chromatin within T cells. This protocol allows scientists to interpret evidence of chromatin decondensation during T cell activation demonstrated by an increase in the mean florescence intensity (MFI) of fluorescent Histone H3 antibodies

Tags

Flow CytometryChromatinT CellsActivationHistone H3Cell ViabilityAntigen PresentationClonal ProliferationChromatin Decondensation

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