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A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure
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A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

DOI:
10.3791/54065-v

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07:37 min

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July 14, 2016

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, , , , ,
1Department of Ophthalmology, School of Medicine,Johns Hopkins University

Chapters

  • 00:05Title
  • 01:00Instrument Preparation
  • 02:36Anterior Chamber Cannulation
  • 05:39Results: Retinal I/R Induces Neuronal Cell Death in the Ganglion Cell Layer and Impairs Retinal Neuron Function
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a procedure for inducing retinal ischemia-reperfusion injury by elevated intraocular pressure in mice. Retinal ischemia-reperfusion injury by elevated intraocular pressure serves to model human pathologies characterized by compromised oxygen and nutrient delivery in the retina, enabling researchers to examine potential cellular mechanisms and treatments for human diseases of the retinal neurovascular unit.

Tags

Retinal Ischemia-reperfusion InjuryMouse ModelIntraocular Pressure ElevationRetinal Neurovascular UnitOxidative StressInflammationSurgical TechniqueAnterior Chamber CannulationHeparin SodiumFive Valve ManifoldNeedle Tubing

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