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Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
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Biochemistry
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JoVE Journal Biochemistry
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

DOI:
10.3791/54488-v

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10:39 min

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November 28, 2016

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, , , , , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Center for Quantitative Sciences,Vanderbilt University School of Medicine, 3Department of Cancer Biology,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 01:13Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 1
  • 03:07Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 2
  • 04:31Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 3
  • 06:05Fast-protein Liquid Chromatography (FPLC) for HDL Purification
  • 08:05Results: Importance of DGUC to Procedure and Small RNA Library Analysis
  • 09:24Conclusion

Summary

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This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.

Tags

HDLLipoproteinSmall RNANon-coding RNADensity Gradient UltracentrifugationBiomarkerCardiometabolic DiseaseHigh-throughput SequencingReal-time PCR

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