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Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
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JoVE Journal Chemistry
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

DOI:
10.3791/54611-v

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10:13 min

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November 12, 2016

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, ,
1Center for Applied Plant Sciences,The Ohio State University, OARDC, 2Department of Plant Pathology,The Ohio State University, OARDC, 3Department of Horticulture and Crop Science,The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster,The Ohio State University, OARDC

Chapters

  • 00:05Title
  • 00:49Sample Preparation
  • 02:30Organic Acid Standard Preparation
  • 04:34Organic Acid Extraction
  • 06:35SCCA Detection Run Execition and Data Collection
  • 08:18Results: Quantification of Organic Acid Levels in Green Coffee Beans
  • 09:35Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.

Tags

Capillary ElectrophoresisOrganic AcidsCoffea ArabicaCoffee SeedsShort Chain Carboxylic Acids (SCCAs)Sample PreparationLiquid Nitrogen GrindingAuthentic StandardsExternal And Internal StandardsCitric AcidMalic AcidAcetic AcidLactic AcidAdipic Acid

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