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Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy
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Chapters
- 00:05Title
- 01:10Plasma Membrane Vesicle (PMV) Generation
- 03:18PMV Content Examination by Confocal Microscopy
- 05:11PMV Gastrocnemius Muscle Injection
- 07:12Results: Representative PMV Imaging and Analyses
- 08:40Conclusion
Age-related diseases are associated with multiple defects in components of the cytoplasm. Here, we present a protocol to prepare plasma membrane vesicles from bone marrow mesenchymal stem cells. This technique could potentially be used as a means of cytoplasm replacement therapy to ameliorate or even reverse age-associated phenotypes.
