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Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
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Bioengineering
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JoVE Journal Bioengineering
Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

DOI:
10.3791/55803-v

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11:53 min

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July 21, 2017

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, ,
1Department of Chemical and Biomolecular Engineering,University of Delaware, 2Department of Materials Science and Engineering,University of Delaware

Chapters

  • 00:05Title
  • 00:56Formulation of siRNA Nanocarriers
  • 02:09Measuring siRNA Release Using Gell Electrophoresis
  • 04:57Measuring siRNA Release Using Fluorescence Correlation Spectroscopy (FCS)
  • 06:12Cell Culture and In Vitro siRNA Delivery
  • 08:15Controlling Gene Silencing in a Spatiotemporal Manner
  • 09:35Results: Prediction and Spatiotemporal Control of Gene Silencing
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

We present a novel method that uses photo-responsive block copolymers for more efficient spatiotemporal control of gene silencing with no detectable off-target effects. Additionally, changes in gene expression can be predicted using straightforward siRNA release assays and simple kinetic modeling.

Tags

Gene SilencingSiRNA ReleasePhoto-responsive Polymeric NanocarriersSpatiotemporal ControlStructure-function RelationshipsStimuli-responsive DeliveryKinetic ModelingGene Expression ControlPhoto-responsive PolymersN/P RatioSDS SolutionUV LaserNanocarrier Solution

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