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Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
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Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

DOI:
10.3791/56018-v

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11:24 min

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February 10, 2018

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, , , , , , , , , ,
1National Institute of Immunology

Chapters

  • 00:00Title
  • 02:05Preparation
  • 03:40Procedure
  • 07:30Results
  • 09:56Precautions

Summary

Automatic Translation

Automatic Translation

We have described a protocol for performing partial hepatectomy (PHx) and cell transplantation via spleen in NOD.SCID (NOD.CB17-Prkdcscid/J) mice. In this protocol, an incision is made to expose and resect the left lobe of the liver followed by another incision for the intrasplenic transplantation of cells.

Tags

Hepatocyte TransplantationPartial HepatectomyNOD.SCID MiceCell EngraftmentCell-based TherapyGFP MouseNeoHep CellsIsoflurane AnesthesiaSurgical Procedure

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