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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
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Chapters
- 00:04Title
- 00:50Preparation for Ovary Dissection and Culture
- 02:13Ovary Dissection and Organ Culture
- 03:34Tissue Fixation
- 04:12Whole Mount Immunofluorescence
- 05:42Ovary Clearing for Imaging
- 06:37Results: Immunofluorescence Imaging of Cleared Ovaries
- 07:23Conclusion
Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.
