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Journal / Bioengineering / Production of Elastin-like Protein Hydrogels for…
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Bioengineering

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JoVE Journal Bioengineering
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
 

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Article DOI: 10.3791/57739-v • 11:46 min • May 19th, 2018
May 19th, 2018 •
*1, *2, 1, 2
1Department of Bioengineering, Stanford University, 2Department of Materials Science and Engineering, Stanford University
* These authors contributed equally

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Summary
Automatic Translation

Recombinant protein-engineered hydrogels are advantageous for 3D cell culture as they allow for complete tunability of the polymer backbone and therefore, the cell microenvironment. Here, we describe the process of recombinant elastin-like protein purification and its application in 3D hydrogel cell encapsulation.

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Keywords: Elastin-like Protein Hydrogel 3D Cell Encapsulation Fluorescent Labeling Confocal Microscopy Biomaterials Regenerative Medicine Cell Biology Cell-extracellular Matrix Interactions Recombinant Protein Expression IPTG Induction Cell Pellet TEN Buffer
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